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horseradish peroxidase  (Vector Laboratories)


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    Structured Review

    Vector Laboratories horseradish peroxidase
    Horseradish Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SA-5014-1/Streptavidin%2C+Horseradish+Peroxidase+conjugated%2C+Concentrate%2C+for+ELISAs+and+Blots/10__1016_slash_j__ejphar__2025__178072-96-5-10
    Average 94 stars, based on 169 article reviews
    horseradish peroxidase - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid
    Article Snippet: Briefly, during Lectin ELISA of serum and blister fluid, biotinylated lectins were diluted with 1% BSA to the following concentrations: HPA-L (Vector, B-1115) 5μg/mL, SNA (Vector, B-1305) 0.5μg/mL, PSA (Vector, B-1055) 0.5μg/mL, SBA (Vector, B-1015) 0.5μg/mL, RCA120 (Vector, B-1085) 0.05μg/mL, AAL (Vector, B-1395) 0.01μg/mL, while during Lectin ELISA of saliva, biotinylated lectins were diluted to the following concentrations: HPA-L 5μg/mL, SNA 2μg/mL, PSA 1μg/mL, SBA 5μg/mL, RCA120 1μg/mL, AAL 1μg/mL. .. Streptavidin linked to HRP (Vector, SA-5014) was diluted 1:2000 and incubated at 37°C for 30 minutes. .. The absorbance (optical density, OD) of each well was read at 450 nm by an automated microplate reader (Bio-Rad 680, Bio-Rad Laboratories, USA).

    Article Title: Targeting of bowel tissue by fully human antibodies cross-reactive with human and mouse GPA33 antigen.
    Article Snippet: Briefly, individual phage clones were incubated on NuncTM MaxisorpTM 96-well plate (Thermo Fisher Scientific, 442404) coated with GPA33. .. Following incubation and washing, the remaining phages on the plate were detected using biotin-conjugated anti-filamentous phages M13 fd F1 antibody (anti-M13 antibody; Abcam, Cambridge, UK, ab20337) and streptavidin horseradish peroxidase (Vector Laboratories, Newark, CA, USA, SA-5014–1) antibodies and 3,3′,5,5′-tetramethylbenzidine (TMB; Fujifilm Wako, 208–17371) as the substrate with the reaction being stopped using 1 M of Sulfuric Acid (Nacalai Tesque, Inc., Kyoto, Japan, 95626–06), and read using a microplate reader (iMark, Bio-Rad Laboratories). ..

    Article Title: An Evaluation of Cation–Chloride Cotransporters NKCC1 and KCC2 in Carbamazepine-Resistant Rats
    Article Snippet: The nitrocellulose membranes underwent a two-hour blocking step with 10% milk powder in PBS with Tween-20 (0.15 M PBS, 0.1% Tween-20) prior to a 48-h incubation with respective primary antibodies, such as rabbit anti-NKCC1 (1:1000; 130 kDa; ab59791, Abcam, Cambridge, UK) and rabbit anti-KCC2 (1:6000; 123 kDa; ab49917, Abcam, Cambridge, UK). .. The membranes were then washed (3 times, 5 min) and incubated for 2 h with the horseradish peroxidase-conjugated secondary antibody: goat anti-rabbit IgG antibody (H + L) peroxidase (HRP) (1:10,000; PI-1000-1, Vector Laboratories Inc., Newark, CA, USA). .. Proteins were detected by a chemiluminescence reaction (LuminataTM Crescendo Western HRP substrate, Millipore Corp, Burlington, MA, USA).

    Article Title: Separation Methods Affect Glycan Patterns in Extracellular Vesicle Preparations From Canine Osteoblasts and Osteosarcoma Cells
    Article Snippet: .. Membranes were then incubated for 30 min at room temperature with HRP‐conjugated Streptavidin (SA‐5014, Vector Laboratories, Newark, California, USA) to detect bound biotinylated lectins. ..

    other:

    Article Title: Blood group O expression in normal tissues and tumors.
    Article Snippet: Goat anti-rat-647 (#112–605-167, 1:400 dilution, Jackson ImmunoResearch) was used to detect OmcFL3-02 and Tn4-31L, while 20 The FEBS Journal (2025) a 2025 The Author(s).



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